GSM5124031: BRG1 ChIP CAL-120 Infigratinib 2d 3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMARCA4
Cell type
Cell type Class
Breast
Cell type
CAL-120
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
breast cancer cells
treatment
Infigratinib_2d
cell line
CAL-120
chip antibody
BRG1
chip antibody manufacturer
abcam
chip antibody catalog number
Ab110641
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were then lysed with 1% SDS lysis buffer (1% SDS, 10mM EDTA (pH=8), 50mM Tris-HCl (pH=8)) containing protease and deacetylase inhibitors, and sonicated for 5 min with settings of PIP 140, DF 5%, cpb 200 on a Covaris E220 (Covaris Inc., Woburn, MA) to obtain 200-600bp chromatin fragments. libraries were constructed from 2 ng of DNA, using the ThruPLEX DNA-seq kit (#R400427, Rubicon Genomics), according to the manufacturer's protocol; finished libraries were quantified by the Qubit dsDNA High-Sensitivity Assay Kit (#32854, Thermo Fisher Scientific), by an Agilent TapeStation 2200 system using D1000 ScreenTape (# 5067-5582, Agilent), and by RT-qPCR using the KAPA library quantification kit (# KK4835, Kapa Biosystems), according to the manufacturers' protocols; ChIP-seq libraries were uniquely indexed in equimolar ratios, and sequenced to a target depth of 40M reads on an Illumina NextSeq500 run, with single-end 75bp reads.